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Family Asteraceae / Compositae
Enydra fluctuans Lour.

Zhao ju

Scientific names Common names
Enydra fluctuans Lour. Kankong-kalabau (Tag.)
Meyera fluctuans (Lour.) Spreng. Common enhydra (Engl.)
  Buffalo spinach (Engl.)
  Marsh herb (Engl.)
  Water cress (Engl.)
Some compilations refer to Enhydra fluctuans as Enydra fluctuans.
Some compilations consider Enhydra fluctuans Lour. and E. anagallis as synonyms; some as separate species.
Enydra fluctuans DC. is an accepted name with no synonyms recorded for the name The Plant List
Enydra anagallis Gardner. is an accepted name with no synonyms recorded for the name The Plant List
Enydra fluctuans Lour. is an accepted name The Plant List
Meyera fluctuans (Lour.) Spreng. is a synonym of Enydra fluctuans Lour. The Plant List

Other vernacular names
BENGALI: Hingcha, Helencha, Hinchashak, Harhach.
CHINESE: Zhao ju.
INDIA: Helencha, Harkuch.
THAI: Phak bung ruem.
VIETNAMESE: Cây rau ngổ, Rau ngổ, Ngổ trâu, Ngổ đất, Ngổ hương.

Kankong-kalabau is a prostrate, spreading, annual herb. Stems are somewhat fleshy, 30 centimeters or more in length, branched, rooting at the lower nodes, and somewhat hairy. Leaves are stalkless, linear-oblong, 3 to 5 centimeters in length, pointed or blunt at the tip, usually truncate at the base, and somewhat toothed at the margins. Flowering heads are without stalks, borne singly in the axils of the leaves, and excluding the bracts, are less than 1 centimeter in diameter. Outer pair of the involucral bracts is ovate and 1 to 1.2 centimeters long; the inner pair is somewhat smaller. Flowers are white or greenish-white. Fruits are achenes enclosed by rigid receptacle-scales. Pappus is absent.

- In the Philippines, found only in Rizal Province in Luzon and occasional along the banks of small streams in and about Manila.
- Certainly introduced.
- Found in tropical Africa and Asia to Malaya.

- Study yielded flavonoids, tannins and saponins.
- Leaf extract study yielded two new chlorine containing melampolids, in addition to three known sesquiterpene lactones.
- Phytochemical analysis yielded alkaloids, saponins flavonoids, triterpenoids/steroid, tannins, carbohydrates, and glycosides.
- Ethyl acetate fraction yielded two flavonoids: baicalein 7-O-glucoside and baicalein 7-O-diglucoside.
- Study showed low ash content and a good source of beta-carotene (3.7 to 4.2 mg/100g on fresh weight basis.

- Study of methanolic extract showed total phenol and total flavonoid contents of 153.08 ± 0.38 mg/mL and 172.04 ± 0.56 mg/mL, respectively. (see study below) (20)

- Leaves are antibilious.
- Demulcent, cooling, laxative.
- Study showed antimicrobial, analgesic, antidiarrheal, antioxidant, anti-cancer, CNS depressant, iron chelating, hepatoprotective, gastroprotective properties.

Parts used
Leaves, young plant parts.


- In Malaya, young parts are used as salad.
- Sometimes steamed before they are eaten.
- In the Philippines, leaves are pressed and applied to the skin as a cure for herpetic eruptions.
- The Malays use the young parts and bitter leaves as laxative.
- Leaves used for diseases of the skin and nervous system.
- Leaf paste applied to inflamed breasts to reduce inflammation.
- In Calcutta, fresh juice of leaves used as adjunct to tonic medicines; used for neuralgia and other nervous diseases. In Indian medicine, also used for various skin diseases and as a laxative.
- In Assam, India, plant juices used for skin diseases, nervous disorders and high blood pressure.
- Expressed juice of leaves used as demulcent in cases of gonorrhea; mixed with cow's or goat's milk.
- As a cooling agent, leaves are pounded and made into paste and applied cold to the head.
- Plant used for torpidity of the liver. Infusion is prepared the previous evening, boiled with rice and taken with mustard oil and salt.
- Used by the tribal practitioners of the Marakh sect of the Garos for treatment of diabetes. (19)
- In Ayurveda, used for liver disorders, skin and nervous disorders; also, as laxative.

Study results showed extract variations, but the methanol extract of A. longifolia, I. aquatica and Enhydra fluctuans showed antimicrobial activity against pathogenic bacteria - S. aureus, P. aeruginosa, E coli and M. luteus. (1)
Antioxidant: Study showed the crude extract to contain flavonoids, saponins and tannins. The ethyl acetate fractions showed the highest free radical scavenging activity
Analgesic: Study evaluating the analgesic activity showed promising results in both acetic acid-induced writhing and the tail-flick methods. (6)
Antidiarrheal: Study of the methanol and aqueous extracts of the whole plant showed antidiarrheal activity on castor-oil induced diarrhea. The methanolic extract moderately inhibited growth of S. dysenteriae, S. boydii and S. flexneri; the aqueous extract inhibited growth of S. aureus, S. dysenteriae and S. boydii. (7)
Flavonoids / Anticancer: Study yielded two flavonoids: baicalein 7-O-glucoside and baicalein 7-O-diglucoside. They were screened for anticancer activity against Erlich/s ascites carcinoma bearing Swiss albino mice. Treatment caused a significant decrease in tumor cell volume and increase of life span. Treatment with the flavonoids was found to be cytotoxic in the in-vitro model. (8)
CNS Depressant Activity: Neuropharmacological effects of three fractions of aerial parts were studied using mice models. Results showed significant spontaneous motility depressant, sedative, anticonvulsant and anti-stress activity. (10)
Flavonoids / Analgesic Activity: Study of total flavonoids in Swiss albino mice showed analgesic and anti-inflammatory activity. The results may be attributed to high free radical scavenging and antioxidant potential of flavonoids in the ethyl acetate fraction of E. fluctuans. (11)
Hepatoprotective / CCl4-Induced Damage: Study evaluated the hepatoprotective potential of EF against carbon-tetrachloride-induced oxidative damage in rats. Results showed the flavonoid rich ethyl acetate fraction to have significant hepatoprotective activity, probably due to the ability of the extract to inhibit lipid peroxidation and increase the anti-oxidant enzymatic activity. (12)
Antimicrobial / Cytotoxicity: Study evaluated three medicinal plants (E. fluctuans, Clerodendrum viscozum and A. peniculata) for antimicrobial and cytotoxic activities against some pathogenic organisms and Artemia salina (brine shrimp nauplii). All three showed activity against brine shrimp nauplii. A methanol leaf extract of E. fluctuans showed significant efficacy against tested bacteria and fungi. (15)
Iron-Chelating / Antioxidant: Chelation of free iron can prevent the formation of free radicals. An antioxidant prevents the activity of free radicals either by inhibition or scavenging of free radicals. Study showed E. fluctuans to have both iron chelating and antioxidant activity. (18)
Anti-Diarrheal / In vitro Antimicrobial Activity: Study of extracts of E. fluctuans showed antidiarrheal activity. The methanolic extract showed moderate inhibitory activity on Shigella dysenteriae, S. boydii and S. flexneri; the aqueous extract inhibited Staphylococcus aureus, S. dysenteriae, and S. boydii. (13)
Phagocytosis Effects / Leaves: Study evaluated the effect of aqueous extract of leaves on neutrophil phagocytic function. Results showed the extract stimulated chemotactic, phagocytic, and intracellular killing potency of human neutrophils at different concentration. probably through stimulation of cell-mediated immune system. (16)
Natural Flavonoids / Anti-Inflammatory / Gastric Cytoprotective: Flavonoid isolated from the leaves of E. fluctuans showed anti-inflammatory activity by inhibiting COX-2 and 5-LOX. Its anti-ulcerogenic effect can be attributed to its action on mucosa defense factors. (17)
Anthelmintic / Antioxidant / Thrombolytic: Methanolic extract was evaluated for anthelmintic and thrombolytic activities. Results showed significant antioxidant activity with IC50 of 135.20  ± 0.56 µg/mL, with reducing power increasing with concentration. Anthelmintic activity of crude extract against Pheretima posthuma showed concentration dependent effect and compared with albendazole. Clot lysis activity of crude extract was significant (31%) compared to standard streptokinase's clot lysis activity of 40.13%. (see constituents above) (20)


Last Update August 2016

IMAGE SOURCE: / Enydra fluctuans, Buffalo Spinach, Water Spinach ....Rau Ngổ, Ngổ Trâu / -- Vietnam Plants & America plants
Phuong Tran
/ Creative Commons Attribution / flickr / Click on graphic to see original image

Additional Sources and Suggested Readings
Antimicrobial efficacies of methanol extract of Asteracantha longifolia, Ipomoea aquatica and Enhydra fluctuans against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus / J N Bhakta, P Majumdar, Yukihiro Munekage /
The Internet Journal of Alternative Medicine™ ISSN: 1540-2584
Anti-inflammmatory activity of Enhydra fluctuans Lour, Leaf. Plaban Bhattacharya, Anup Maiti, Subhash C. Mandal. The 9th International Congress on Ethnopharmacology at Naning, P.R. China. (2006).
Hepatoprotective nature of Enhydra fluctuans leaf on Paracetamol induced hepatic damage / Plaban Bhattacharya, Anup Maiti, Subhash C. Mandal. The 94th session of the Indian Science Congress at Annamalainagar, India.(2007).
Wild plants of Majuli Island and Darrang districts of Assam / Indian Journ of Traditional Knowledge, Vol 6(1), Jan 2007, pp 191-194

Sesquiterpene lactones from Enhydra fluctuans / N R Krishnaswamy and Niranjan Ramji /
Volume 38, Issue 2, January 1995, Pages 433-435 / doi:10.1016/0031-9422(94)00705-X
Analgesic activity of Enhydra fluctuans / M T Rahman, N Gegum et al / Fitoterapia, Vol 73, Issues 7-8, December 2002, Pages 707-709 / doi:10.1016/S0367-326X(02)00212-5
Evaluation of Anti-diarrheal Activity of Enhydra fluctuans / S J Uddin, M M Ferdous, R Rouf et al / J Med Sc, 5 (4), 324-327
Flavonoids of Enhydra fluctuans exhibit anticancer activity against Ehrlich's ascites carcinoma in mice. / Sannigrahi S, Mazumder UK, Mondal A, Pal D, Mishra SL, Roy S. / Nat Prod Commun. 2010 Aug;5(8):1239-42.
Sorting Enydra names / Maintained by: Michel H. Porcher, / MULTILINGUAL MULTISCRIPT PLANT NAME DATABASE / A Work in Progress. School of Agriculture and Food Systems. Faculty of Land & Food Resources. The University of Melbourne. Australia / Copyright © 1997 - 2000 The University of Melbourne.
Pharmacological Evaluation of Enhydra fluctuans Aerial Parts for Central Nervous System Depressant Activity
Sudipto Kumar Roy et al / Pharmacologyonline 1: 632-643 (2011)
Flavonoids of Enhydra Fluctuans exhibits analgesic and anti-inflammatory activity in different animal models /
Sannigrahi S, Mazumder UK, Pal D, Mishra ML, Maity S. / Pak J Pharm Sci. 2011 Jul;24(3):369-75.
Hepatoprotective Potential of Flavonoid Rich Fraction of EnhydraFluctuans Against CCl / Sannigrahi et al / Pharmacologyonline, 2: 575-586 (2009)
Evaluation of Anti-diarrhoeal Activity of Enhydra fluctuans / S.J. Uddin, M.M. Ferdous , R. Rouf , M.S. Alam , M.A.M. Sarkar and J.A. Shilpi / Journal of Medical Sciences, 5: 324-327. / DOI: 10.3923/jms.2005.324.327
Chemical composition of two semi-aquatic plants for food use. / Dewanji A, Matai S, Si L, Barik S, Nag A. / Plant Foods Hum Nutr. 1993 Jul;44(1):11-6.
Antimicrobial and Cytotoxic Activity of Three Bitter Plants Enhydra Fluctuans, Andrographis Peniculata and Clerodendrum Viscosum / M. Ruhul Amin, Ripon Mondol, M. Rowshanul Habib, and M. Tofazzal Hossain * / Adv Pharm Bull. 2012; 2(2): 207–211 / doi: 10.5681/apb.2012.032
Effect of Enhydra fluctuans Lour. Leaf Extract on Phagocytosis by Human Neutrophils / K. S. Patil, P. Majumder, R. R. Wadekar / Journal of Natural Remedies, Volume 8, Issue 1, January 2008
Natural Flavonoids Isolated From the Leaves of Enhydra fluctuans Inhibits Cyclooxygenase-2 and 5-Lipooxygenase Inflammation in Various Models / Satyajit, D. Pradhan / International Journal of Research in Pharmacology and Pharmacotherapeutics
In Vitro Studies on Antioxidant and Iron-chelating Activity of Enhydra fluctuans Lour / L.N. PATRALEKH, * G. MUKHERJEE ** / Sci. & Cult. 76 (11–12) 537–539 (2010)
MEDICINAL PLANTS USED FOR TREATMENT OF DIABETES BY THE MARAKH SECT OF THE GARO TRIBE LIVING IN MYMENSINGH DISTRICT, BANGLADESH / Mohammed Rahmatullah, Md. Nur Kabidul Azam, Zubaida Khatun, Syeda Seraj, Farhana Islam, Md. Atiqur Rahman, Sharmin Jahan, Md. Shah Aziz, Rownak Jahan / African Journal of Traditional, Complementary and Alternative medicines (AJTCAM), Vol 9, No 3 (2012)
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.
Sourov Kuri, Md. Mustahsan Billah,* S. M. Masud Rana, Zannatul Naim, Md. Mahmodul Islam, Md. Hasanuzzaman, Md. Ramjan Ali, and Rana Banik / Asian Pac J Trop Biomed. 2014 Apr; 4(4): 299–305.
doi: 10.12980/APJTB.4.2014C677

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