Labanos is a coarse, annual crop plant. Roots are fleshy, pungent
and variable in size and form. Leaves are roughly hairy, the lower ones lyrate. Flowers are variable, about 1.5 centimeters long, usually white
or lilac, with purple veins, sepals erect, lateral ones saccate
at the base. Pod is indehiscent, lanceolate, cylindrical, and 2 to 2.6 centimeters in length, and terminates in a long beak. Seeds are separated by pith.
- Widely cultivated in the Philippines at all altitudes.
· Considered anthelmintic,
antifungal, antibacterial, antiscorbutic, diuretic, laxative, tonic,
carminative, corrective, stomachic, cholagogue, lithotriptic, emmenagogue.
· The juice of the fresh root is considered powerfully antiscorbutic.
· Roots considered carminative and corrective.
· Flowers considered becnic and cholagogue.
· Seeds considered diuretic, laxative, stimulant, and lithotriptic.
· In Iranian traditional medicine, seeds are considered diuretic
carminative, anti-fever, antitussive and gastric tonic.
• Phytochemical study yielded triterpenes, alkaloids, flavonoids,
tannins, saponin and coumarins.
• Study for volatile constituents yielded 10 isothiocyanates, seven aliphatic hydrocarbons and some other volatile substances.
• Root yields raphanol, rettichol, volatile oil, methylmercaptan, vitamins B1, sinapin and oxydase.
• Seeds yield fatty oil (30%), ash (3.5%), volatile oil, sulphuric acid, erucic acid and C8H15NS2.
• Methanol extraction yielded two new compounds identified as β-sitosterol and 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyltetracos-15'-enoylamino]-8-octa-decene-1, 3, 4-triol. (28)
• Fractionation of methanol extract of seeds yielded seven 4-methylthio-butanyl derivatives, viz., sinapoyl desulfoglucoraphenin (1), (E)-5-(methylsulfinyl)pent-4-enoxylimidic acid methyl ester (2), and (S)-5-([methylsulfinyl)methyl]pyrrolidine-2-thione (3), together with four known compounds, 5-(methylsulfinyl)-4-pentenenitrile (4), 5-(methylsulfinyl)-pentanenitrile (5), sulforaphene (6), and sulforaphane (7). (see study below) (31)
• Fresh vegetable yields 91.00% moisture; seeds on extraction with petroleum ether yield albuminoids 18.00%, soluble carbohydrates 52.66%, woody fiber 9.34%, and ash 16.00%. (Nadkarni, 1954) (32)
• Roots yield alkaloid and nitrogen compounds: pyrrolidine, phenethylamine, N-methylphenethylamine, 1,2- pyrrolidin-tion-3-il-3-acid-caroxilic-1,2,3,4-tetrahydro-ß-carbolin, and sinapine[3,4,5]. Total amino acids were 0.5% of dry weight; proline (0.5%) was the major constituent, while methionine and cystine were present in trace amounts (0.02%). (39)
• Roots yielded four major organic acids: oxalic, malic, malonic, and erythorbic acid. Lipid total was 1.23%. Major fatty acids in seed lipids were erucic, oleic, linoleic, and linolenic acids. (39)
• Study of seed oil by Soxhlet extraction yielded oil percentage of 33.4% by weight. GC-MS analysis yielded nine major components viz. palmitic acid (5.52%), oleic acid (25.22%), linoleic acid (12.02%), 11-eicosenoic acid (10.66%), eicosanoic acid (1.90%), erucia acid (32.79%), heneicosanoic acid (2.68%), nervonic acid (2.91%) and tetracosanoic acid (1.84%). (41)
• Leaf extract yielded photosynthetic pigments (3.40 mg/g fresh weight), ascorbic acid (0.000429 mg/g fw) and foliar phenol content (0.0270 mg/g fw), together with alkaloids, flavonoids, glycosides, tannin, phenolic compounds, triterpenoids and steroids. Root extract yield phtosynthetic pigments (0.38 mg/g fw), ascorbic acid (0.0000825 mg/g fw) and foliar phenol (0.0375 mg/g fw) along with alkaloids, glycosides, triterpenoids and steroids. (see study below) (49)
· Whole plant.
· When seeds are ripe, harvest the whole plant, sun-dry, remove
the seeds and dry again. Crush on use. Roots can also be sun-dried for
Edibility / Nutrition
Leaves, flowers, roots,
and seeds are edible.
- A popular, common, and inexpensive vegetable, eaten raw or cooked.
- Young leaves are also eaten raw or cooked.
- Excellent source of iron, ascorbic acid, folic acid, and potassium; a good source of vitamin B6, riboflavin, magnesium, copper and calcium.
· For diarrhea: boil
the fresh leaves to concentrated decoction and drink.
· Juice of leaves increases the flow of urine and promotes bowel
· Juice of fresh leaves also used as laxative; also for dropsy and general anasarca.
· Root considered stimulant; also used for piles and stomach pains.
· Juice used to expel wind from the bowels.
· Juice of fresh roots considered antiscorbutic.
· Roots are crushed and applied locally as dressing or poultice for burns, scalds, ecchymoses, or fetid or smelly
· Decoction of root used for fevers.
· Decoction of roots used to bring out the rash in eruptive fevers.
· Coughs: Decoction of flowers; or, boil 6 to 15 gms seed preparation
to decoction and drink.
· Seeds promote the flow of urine, bowel movements, and menstruation.
· Seeds used for cancer of the stomach.
· For patients with edema, bloated belly (ascites), pale yellowish
face, and oliguria: used dried root preparation with citrus rind preparation
(5:1 proportion). Boil to a concentrated decoction and drink.
· In Mexico, black radish has been used for treatment of gallstones and for decreasing blood lipids.
· In India, plant used as purgative, stimulant, antiscorbutic, diuretic and lithotryptic. Roots used for piles, gastric pains, dysuria and strangury. Seeds used as expectorant, diuretic, laxative, and carminative. (32)
· In Iranian traditional medicine used as laxative, antitumor, antiproliferative, and abortifacient. (42)
Pharmacological basis for the gut stimulatory activity of Raphanus sativus
leaves: A study on the crude extract of RS leaves showed the presence
of a histaminergic component plus a weak spasmolytic factor supporting
its traditional use for constipation. (1)
• Toxicity Report: Severe Toxic Hepatitis Provoked by Squeezed Black Radish
(Raphanus Sativus) Juice - Case Report: Cited in phytotherapy
literature as a plant with hepatoprotective properties, this reports
a severe toxic hepatitis from use of black radish extract to dissolve
bile duct stone. (2)
• Hepatoprotective: Studies on Raphanus sativus as Hepatoprotective Agents (Thesis): Results
showed the ethanolic extract of RS contain hepatoprotective constituents.
Activity / Diuretic: Study of aqueous extract
of the bark of RS on rats showed a significant decrease in the weight
of stones. Study also showed an increase in 24 hour urine volume compared
to control. (4)
• Water Phenol
Decontamination: Decontamination of Water Polluted with
Phenol Using Raphanus sativus Root: Plant materials have been used in
decontamination of water polluted with phenolic compounds. The study
used RS roots (root juice and pieces). Results showed good phenol removal
from aqueous solutions with cut R sativus root and juice. (5)
Antioxidant / Lipid Peroxidation Inhibition:
Study of methanol extract of RS showed inhibition of lipid peroxidation
in vivo and in vitro, providing protection by strengthening antioxidants
like glutathione and catalase. Results suggest inclusion of the plant
in every day diet may be beneficial. (6)
Phytochemicals / Toxicity Study / Hepatoprotective Activity:
Study of showed carbon tetrachloride induced hepatotoxicity
was reduced by the plant as showed by inhibition of increased liver
enzyme activities and bilirubin concentration together with histopath
changes. Toxicity study showed no adverse effect on livers. Phytochemical
studies yielded triterpenes, alklaoids, flavanoids, tannins, saponins
and coumarins. (7)
Phytochemicals / Gastroprotective: Study of
the freshly squeezed radish juice for its anti-gastric ulcer activity
in experimental models showed it possessed gastroprotective potential
related to mucus secretion stimulation and an increase in nonproteinsulfhydryl
(NP-SH) concentration, probably due to prostaglandin-inducing abilities
mediated through antioxidant activity. Phytochemicals study yielded
flavonoids, anthocyanins and sulfurated constituents. (8)
• Antioxidant / Choleretic: Study of extract from radish sprouts in rats showed antioxidant properties and significantly induced bile flow. (10)
• Anti-Diabetic: Study showed that the sprouts of Japanese radish has the potential to alleviate hyperglycemia and may serve i the primary prevention of diabetes mellitus. (13)
• Spasmogenic Effect: In vitro study was done to evaluate the effects of crude extracts of roots on isolated rat trachea. Results showed significant cholinergic spasmogenic effects.
• Hepatoprotective / Carbon Tetrachloride Toxicity: Study of a crude extract of seed showed hepatoprotective effect against liver damage induced by CCl4. (16)
• Antilithiasic / Hypolipidemic Effect: Study evaluated the effect of juice squeezed from black radish root in cholesterol gallstones and serum lipids in mice. A lithogenic diet induced cholesterol gallstones and increased cholesterol and triglyceride levels. Juice treatment caused significant eradication of cholesterol gallstones, together with decrease in cholesterol and triglycerides., with an increase in HDL. (17)
• Antimicrobial / Root Juice: Study evaluated R. sativus root juice for antimicrobial potential against five bacterial strains, viz. Klebsiella pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, and Escherichia. Results showed considerable antimicrobial activity against all tested microorganisms. (18)
• Antibacterial / Seed Extract: Study evaluated the potentiality of different solvent extracts against various pathogenic strains, viz. E coli, K pneumonia, P vulgaris, P aeruginosa, Shigella sonnie, S typhi and S paratyphi. The highest activity was seen in ethanol and methanol extracts. The effect could be secondary to extracted active compounds like flavonoids, phenolic compounds, saponins, and other secondary metabolites. (19)
• Anti-Inflammatory / Root Extract: Study evaluated the effect of a root extract on anti-inflammatory activity in rats using a carrageenan induced paw edema model. The hydroalcoholic extract showed potent anti-inflammatory activity which may be due to the presence of flavonoids, phytosterols, and tannins and also inhibition of inflammatory mediators ( histamine, serotonin, prostaglandins, bradykinin, substance P, etc.)
• Hepatoprotective / CCl4-Induced Toxicity: Study evaluated the hepatoprotective activity of radish enzyme extract in vitro and in vivo test. Results showed the enzyme extract can significantly diminish hepatic damage by toxic agents such as tacrine or CCl4. (21) Study of crude extract of seeds in doses of 600 and 800 mg/kg may be protective against liver damage caused by CCl4. (22)
• Antidiabetic / Root Juice: Study of R. sativus root juice for glycemic attributes showed good hypoglycemic potential coupled with antidiabetic efficacy. (23)
• Laxative / Leaf: Study evaluated aqueous extract and fresh juice for laxative action using wistar albino rats in various experimental models such as loperamide induced constipation, laxative activity test, gastrointestinal motility test and water and electrolyze secretion test. Results showed significant laxative activity at higher dose of 750 mg/kg. (24)
• Anti-Inflammatory / Leaf and Root Juice: Study evaluated freshly squeezed leaf and root juice in for anti-inflammatory activity in albino rats. While both leaf juice and root juice significantly reduced carrageenan and formalin induced paw edema in rats, the root juice produced more significant anti-inflammatory effects in both acute and chronic models of inflammation. However, the effect was less than standard drug diclofenac sodium. (25)
• Antinociceptive / Roots: Study evaluated the antinociceptive potential of methanolic extract of roots in intraperitoneally administered acetic acid induced pain model in mice. Results showed significant antinociceptive activity, with the highest extract dose nearly comparable to the highest dose of aspirin. (26)
• Triglyceride Effect: Study of aqueous extract showed lowering of plasma triglyceride, but had no effect on plasma glucose or cholesterol. (27)
• α- Amylase and α- Glucosidase Inhibitory Activity: Study of ethanolic extract and fractions showed dose dependent inhibition of α-amylase and α-glucosidase enzyme, exhibiting lower inhibitory activity than acarbose. Results suggest potential for antidiabetic therapy and development of medicinal preparations, nutraceuticals, and function foods for diabetes. (29)
• Glucocerebroside / Anti-Cancer: Methanol extraction yielded two compounds determined to be 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyl-tetracos-15'-enoyl amino]-8-octa-decene- 1, 3, 4-triol (glucocerebroside). The glucocerebroside could inhibit the growth of BEL-7402 cancer cells and induce apoptosis in these cells. (30)
• 4-Methylthio-butanyl Derivatives / Seeds / Anti-Cancer / Anti-Inflammatory: Study evaluated seed extracts for anti-inflammatory and antitumor activities. Fractionation yielded seven 4-methylthio-butanyl derivatives. Compound 1 (sinapoyl desulfoglucoraphenin) inhibited nitric oxide production. All compounds showed antiproliferative activities against four human tumor cell lines. (see constituents above) (31)
• Cardioprotective / Antioxidant: Study evaluated water and ethanolic extracts of fruit powder for cardioprotective activity in Cyclosporin-induced ischemia in rabbits. The powder and aqueous extract significantly decreased (P<0.001) the uric acid and activity of enzymes (SGOT and LDH) in treated rabbits. Both fruit powder and aqueous extract showed dose-dependent in vitro free radical scavenging effect on DPPH assay. (33)
• Antifertility Effects: Study on R. sativus showed antifertility activity. In male rates, study showed a decrease in sperm count, motility, and weight of testis and epididymis. In female rats, it disturbed the estrous cycle and decreased the number of implantation, average number of pups delivered, average weight of the pups, number of corpus lutea, and weight of ovary. Results suggest a potential as antifertility agent. (34)
• Phytoremediation / Copper: Based on bioaccumulation coefficient (BAC) analysis, mustard and radish can be considered high accumulator plants for Cu. Radish has been shown to produce 10 times more biomass than the other three plant studies, and accumulation of copper was higher in the root tissue of radish and mustard. (35)
• Analgesic / Anti-Inflammatory / Leaves: Study evaluated the analgesic (hot plate and tail immersion) and anti-inflammatory (carrageenan) activities of R. sativus leaves in animal model. Results showed significant (p<0.05) dose-dependent analgesic and anti-inflammatory activities. (36)
• Phytoremediation / Lead: Pot culture experiments using radish investigated lead (Pb) phytotoxic effects on antioxidant enzymes and other early warming biomarkers of soil Pb exposure. Results showed radish is a hyperaccumulator plant that can concentrate heavy metals in different parts, with potential use for remediation of polluted areas. (37)
• Anticarcinogenic / Galactan / Colon Cancer: Study evaluated the anti-carcinogenic effect of Raphanus sativus in combating chemically (DMH) induced colon cancer. Results showed RS significantly reduced serum CEA (p<0.01) and CA19-9 (p<0.01) as evidence of anticarcinogenic effect. Results showed the galactan polysaccharide of RS has pronounced cytotoxic effects on colon cancer cell line and might be a suitable candidate as chemopreventive and adjuvant therapy for colon cancer. (38)
• Insecticidal: Study evaluated four medicinal plants, Peganum harmala, Ajuga iva, Aristolochia baetica and Raphanus raphanistrum (aerial parts) for insecticidal effects on stored grain pest Tribolium castaneum. Results showed all extracts disrupted the development cycle of the insect and suggests potential for managing populations of T. castaneum. (40)
• Comparative Extraction of Quercetin from Leaves: Quercetin is the most important flavonoid in the plant. Study reported on the optimized condition for quercetin extraction from leaves, which is due to promoting larger agitation (cavitation) in UAE (Ultrasonic Assisted Extraction) method. (42)
• Antidiabetic / Leaves: Study evaluated different solvent extracts of R. sativus leaves for antidiabetic activity using alloxan induced diabetic rats. The aqueous extracts showed statistically significant protective activity and maximum reduction in blood glucose (p<0.001) in comparison to standard glibenclamide. (43)
• Green Synthesis of Silver Nanoparticles / Peels: Study reports on the economic, eco-friendly, and clean synthesis of silver nanoparticles using Raphanus sativus peel extract as reducing and stabilizing agent. (44)
• Effect on Enterophathogenic E. Coli In Vitro and In Vivo / Lactobacillus acidophilus and Seeds: Study evaluated the antibacterial activity of ethanol extracts of radish seeds and L. acidophillus against Enteropathogenic E. coli in vitro and in vivo mice model. Results showed inhibition of E. coli growth by ethanol extract of L. acidophilus. Results confirm the beneficial effects of L. acidophilus as probiotic and the ethanol extract of radish seeds as treatment compound for diarrheal diseases caused by bacterial pathogens. (45)
• Silver Nanoparticles / Peels / Antifungal / Insecticidal: Study evaluated the antifungal and insecticidal activity of biologically synthesized silver nanoparticles using peel extracts. Peel extracts were used for reduction of Ag ions and capping the synthesized nanoparticles to avoid further agglomeration. Results showed significant toxicity (LC50 of 7.61 ppm/48h) over mango leafhopper, Amritodus brevistylus. It was also effective against fungal pathogen, Aspergillus niger, with a strong inhibitory zone of 80mm. (46)
• Hepatoprotective / Paracetamol Toxicity / Roots: Study of methanolic extract of R. sativus roots showed a protective effect on paracetamol-induced hepatotoxicity in a dose-dependent manner. Results suggest the involvement of antioxidants like glutathione and catalase in the root extract providing protection against paracetamol-induced lipid peroxidation and hepatotoxicity. (47)
• Prevention of Cholesterol Gallstone Formation: Study showed Raphanus sativus L. var. niger may have antilithogenic properties for the prevention of cholesterol gallstones, regulating components in bile through modulation in expression of biliary transporters. (48)
• Antioxidant / Leaves / Roots: Study showed the leaf to have potent antioxidant ability of 78.17% at 200 µg/ml concentration and IC50 value at 122.33 µg/ml concentration while the roots showed maximum % inhibition of 58.38 at 200 µg/ml aqueous extract with IC50 of 166.79 µg/ml concentration. (see constituents above) (49)
• Cardiotoxicity / Leaves: Study in adult male albino rats investigated the cardioprotective effect of R. sativus leaf extracts against myocardial injury induced by isoproterenol (ISO) based of biochemical and histological parameters. Results showed R. sativus leaf extract increase the cardiotoxicity alone and in ISO treated rats. Effect could be due to the histaminergic activity leading to increased myocardial biomarkers. (50)
• Antiurolithiatic / Leaves: Study evaluated the dried powder of leaves of R. sativus for antiurolithiatic activity on urolithiasis experimentally induced by ethylene glycol in male Wistar albino rats. Results showed treatment reduced and prevented urinary stone formation. The mechanism might be related to a diuretic effect and lowering of urinary concentrations of stone forming constituents. Also, the protective effect against oxalate induced lipid peroxidation may contribute to recovery of renal damage. (51)
• Degradation of Methylene Blue by Nanoparticles / Leaves: Study showed silver nanoparticles synthesized by using the plant leaf of R. sativus showed magnificent photocatalytic activity against methylene blue dye molecules and has potential for utilization in water purification systems. (52)
Commercial cultivation; ubiquitous in market places.